DIY Entomology Equipment

This post was written as a guest post for Myrmecos.net, but I though I would post it here as well. 

Flipping through the glossy pages of a Bioquip catalogue, you would think that entomology is a rather expensive pursuit. But, it doesn’t have to be. As an amateur entomologist, I’ve never had money to blow on equipment. Therefore, the majority of my collecting arsenal is home made from commonly available materials. I thought that I would share some of my creations here in the hope that will help others plunge into this exciting hobby without breaking the bank.

Let’s start with collecting equipment. When gathering small insects, such as ants, it is all to easy to accidentally squish your prize. That’s where an aspirator comes in. Though this is not a particularly expensive tool to buy, it is possible to make your own.

As you can see, the mechanism is quite simple. Two pieces of clear vinyl tubing are stuck through holes in the lid of a small jar or vial, and one is covered with a thin piece of cotton to prevent you from inhaling the insects you’re collecting. I used a small piece of cotton from a makeup remover pad, but something thinner would be easier to suck through. For example, fine wire screen or a square from an old pair of tights might work better. Just make sure the holes are small enough! To use it, you point the smaller tube at an insect and suck hard on the other. This pulls your query through the tube and into the vial, where they can be easily collected. This design was inspired the aspirator sold by Bioquip, and operates on the same principles.

Next up is one of my favorites, the Berlese Funnel. It is a device that uses heat to drive arthropods out of soil or leaf litter. These things are usually quite expensive, but I designed and built one for around $25. For complete instructions, you can visit instructables.com, where I’ve written a complete tutorial. To use it, litter is placed onto the screen in the tractor funnel and the light turned on. Any insects that are present attempt to escape the heat of the lightbulb, only to fall through the screen and into a jar waiting below. My design is based loosely on this image form the USDA

In the summer, when you turn a light on outside is is sure to draw an assortment of nocturnal insects. We can take advantage of this positive phototaxis using a device called a light sheet. To make this one, You need a portable light fixture, a light that fits in that fixture (I use a CFL black light which I found at Home Depot, because black lights attract more insects), a length of rope, clothespins, an old white sheet, and, if you aren’t close to an electrical outlet, a battery and inverter to power the light. First, tie the rope between two trees or posts or trees at a height of about six feet. Then, use the clothespins to suspend the sheet from the rope, making sure that it touches the ground with some slack. Finally, hook up your light and point it at the sheet so that is is illuminated. Night flying insects, such as moths and beetles, will be attracted to the light and land on the sheet. Some will hit it and slide down, which is why you need some slack at the bottom to catch them before they disappear into the grass. I first read about this method here

So, you’ve managed to collect your insects. Now what? Well, many make excellent short or long term pets, which gives you the opportunity to closely observe their behavior and life cycle. Most can be kept in simple setups, such as jars or terrariums. For many common insects, there are resources on the web detailing their care and feeding. Or, if you’re a little more adventurous, you can try to rear a more obscure insect using available information on its habitat and diet. One useful device for raising beetles and other insects with soil or wood dwelling larvae is a cardboard rearing box. It consists of, as its name suggests, a cardboard box with a tight fitting lid and a clear jar extending from a hole in its side. Material containing the larvae is place in the box and the box closed. When the adults emerge, they will likely be attracted to the light entering through the jar and accumulate there. However, not all adults will be attracted to light, so it is important to periodically check their progress.

Currently, I am using this box to raise Fire Colored Beetles from larvae I collected late November in Indiana. Since they have a tendency to cannibalize each other, I have placed each larva in its own ziplock bag full of rotting bark. This also keeps the medium moist, as it would be in the wild. Once they near adulthood, I will open the bags so that the beetles can reach the jar as intended.

If you want to permanently preserve the insects you find, then you will probably end up starting a pinned collection. However, before you can pin an insect it has to be dead. This is easier said than done, as many insects are quite delicate and liable to damage themselves if not dispatched correctly. One of the simplest ways to do this is by using a killing jar. To make one, first pour about an inch of plaster of paris into a jar with a tight lid and let is thoroughly dry. Then, add just enough rubbing alcohol or nail polish remover to dampen the plaster. Finally, stick a loosely wadded paper towel into the jar. This will soak up excess fluid and give the insects a place to hide. An insect place in the jar will quickly suffocate without inflicting serious damage upon itself. It can then be removed and pinned for your collection

Moths and butterflies will need to be spread before they dry in order to display their wings. A simple spreading board, pictured below, can be made from pieces styrofoam. The top pieces with the groove between them are held down with pins so that they can be adjusted based upon the size of the insect to be pinned.

If you don’t immediately spread an insect, it will become too brittle to work with. In order to reverse this, the specimen must be relaxed by placing it in a very humid environment. This can be achieved using an airtight box with a few inched of moist, milled peat moss in it. On top of the bed of peat is a sheet of styrofoam on which the insect is placed. A specimen left in the box will absorb the moisture from the air and become pliable. However, depending on the size of the insect, this can take one to several days. Try not to leave it in the box too long though, as mold is liable to develop.

Last but certainly not least is a place to keep your collection. The best option is a professional Shmitt Box, but if that is out of your price range there are a number of suitable DIY alternatives. However, please note that the latter will not protect you collection nearly as well as the former. The simplest would be a shallow box with a tight lid and a layer of styrofoam glued to the bottom. However, what’s the fun in keeping your collection hidden away in a drawer? So, last year I built a pair of glass fronted boxes to house my collection. I will not go into detail here about the construction, but if you want to build one try searching for shadow box plans.

If you do decide to keep your collection in a home made box, it is a good idea to periodically freeze your collection to keep any possible pests under control. Mothballs are also an effective deterrent, but tend to disappear quickly if you box is not entirely air tight.

The back of the box consist of a sheet of hardboard with insulation foam glued to it. Over the foam is a piece of poster board cut to fit the box.

As I have demonstrated here, most of the equipment needed to collect insects can be constructed using a few commonly available items and a whole lot of creativity. Below I’ve included a few links that feature other devices that I hope to construct in the future.

Links:

Collecting and Preserving Insects and Mites: Tools and Techniques: This is a great resource that includes plans for a number of devices as well as general insect collecting information

Manitoba Trap : This site provides instructions for building a Manitoba Trap, which is used to collect horseflies

Aquatic Trap: I haven’t tried this trap, but is extremely simple to build and appears to have worked quite well for the author

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Flora and Fauna of Bloomington IN

Sorry about the lull in posts. The semester is drawing to an end which is always a busy time. This makes it doubly difficult to blog, as I lack both time to write and time to do things worth writing about. Luckily, however, I was able to go exploring in the woods up in Indiana over Thanksgiving break. All around my grandparents house are steep, stream-cut valleys. These areas are forested mostly with hardwoods ranging in size from saplings to giant oaks, though by then all of the leaves had already fallen. here’s a topographic map of the area.

The red dot is the house where I stayed. To the southeast you can see the border of the Morgan Monroe State Forest.

It was a warmer than usual for that time of year, but still cold enough that pretty much every sensible arthropod was holed up under a rock somewhere to wait out the winter. So, I started by searching the streams. The most common inhabitants were little freshwater isopods (relatives of rolly-polies), but small leeches were also present in some slower moving pools.

Leeches!

I also found a hellgramite, an insect deserving of its name. It is the larva of a Dobsonfly, an equally frightening creature. Dobsonflies spend the vast majority of their life, up to three years, underwater. As larvae, they are ambush predators of other aquatic insects. They use their strong jaws to nab passing prey, and can use those same jaws to inflict a painful bite if harassed. Once large enough, the larvae pupate and then emerge as adults which will live only a few days.

While walking in the stream, I stopped to pull apart the layers of green sandstone that lined the banks in the hope of uncovering fossils. I didn’t find any, but I did come across a queen ant that had holed up among the rocks. I collected her in hopes of starting a colony, and brought her back to the house for photographs. I later posted the pictures on bugguide, and learned that she was a Lasius claviger queen. L. claviger is a social parasite of other Lasius species. After the mating flight, she slips into an established nest, kills the queen, and takes her place. That pretty much ruled out starting a colony with her, but I kept her anyway for my collection.

I came up with a new technique to do photos on a white background. I'll have to write about it when I have time.

I also found an established colony of Camponotus americanus, a soil dwelling carpenter ant common across much of the eastern half of North America. The colony was pretty slow due to the cold, and happy to pose for a few pictures in the evening light.

Last but not least, I unearthed a number of Fire Colored Beetle (Dendroides) larvae in a rotten log. These guys are specially adapted to live in the tight space between the wood and bark of rotting trees, as evidenced by their flattened bodies. Their diet is not entirely agreed on, but is though to include rotting wood and fungi, as well as other insects when food becomes scarce.

I brought a few home to rear into adults. I filled five sandwich sized ziplocks with bark from the tree where I found them, placing it so that the undersides were pressed together. I then placed one larvae in each bag, sealed the bags, and set them in rearing boxes, which are just shoeboxes with a jar stuck through the side. This way, when the beetles emerge they will be attracted to the light and end up in the vial where they are easily collected. Of course, I will have to open the bags as that time approaches. Thus far, they seem to be doing well, but none have pupated.

A simple rearing box. These can also be used to separate material collected with a sweep net.

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The Chicken that Lays the Chocolate Eggs

Well not really, but they sure look like it. One of the marans started to lay a couple of days ago, which was quite surprising. I hadn’t expected to see any eggs out of them until next spring. But hey, I’m not complaining!

Ooooooh. Ahhhhhhh.

I figured that, as this was my first maran egg, I out to save it. Since eggs don’t last particularly long in their natural state, I preserved it by blowing. Here’s how it’s done in case you were wondering. First, I gathered my materials: my precious egg, a sewing needle, and a cereal bowl.

Next, I poked a small whole in the top of the egg with the needle.

 And a larger one in the bottom. I used the needle to scramble the yolk up a little bit.

Once I had made my holes, I washed the egg. Then, I put my mouth to the small whole and blew hard, forcing the egg’s innards out of the other end and into the cereal bowl. Just a quick disclaimer, it is generally agreed that raw eggs are bad for you. If you do this, you should probably use a syringe or something of that sort rather than your mouth.

Anyone for scrambled eggs?

After a quick wash, my souvenir was ready for display. It would be cool to do one of these for all of the different colored eggs that my hens lay.

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2 Peas in a Pod

The pea that I cross pollinated a couple of weeks ago seems to be developing as scheduled. It’s a little slow going, but the plant that it’s on is a bit small, which likely contributes.

In fact, if you look closely, you can see the shadow of the little peas developing inside. I only hope that they will be able to mature before a hard frost.

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Pea Breeding Mark II

Way back in 2009, I mused about breeding peas in my garden. I haven’t had much luck though, partly because I haven’t had much success growing, let alone breeding, peas. That is, until this year. I have managed to raise about half-dozen each of blue podded peas and golden sweets, and a couple of days ago I attempted to cross them.

young flower, ready to be pollinated

So first, a bit of background pea anatomy. Pea flowers are self pollinating, with both male organs (stamens) and female organs (stigmas). This makes cross pollinating a bit tricky. If the timing is off, the pea will self pollinate before it can be cross pollinated. To get around this, you need to pull out the flower’s stamens before they shed their pollen.

When cross pollinating, you need two flowers: a father and a mother. The mother needs to be a very young so that it hasn’t self pollinated, such as the one above. The father, on the other hand, should be mature so that it has a good store of pollen, as the one below. Once I had my flowers chosen, I opened up the younger one by peeling back the petals. I then removed the stamens to prevent self pollination. Next I gathered some pollen from a mature flower (below) into a petri dish.

Mature flower. Note the rich yellow pollen.

Once I had my flower and pollen prepped, I slathered a little bit of pollen onto the stigma of the younger flower (see below). If I did it right, a little pea pod will start to develop within the next couple of days, and the peas inside will be hybrids. I can then grow them out next spring, collect their seeds, and begin selecting for new traits!

An emasculated golden sweet flower pollinated with blue podded pea pollen.


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Ant Aphid symbiosis

I’m sorry about the lull in posts lately. I’m taking a lot of hard classes this year, and I haven’t much time to post, let alone do anything blog-worthy.  However, despite my limited time, I did manage to get out in the garden and take a few photos last week, and this was one of my favorites. It depicts an ant tending a colony of yellow aphids. Also visible beneath the ant’s feet is some kind of larva, perhaps Lepidoptera, but I don’t really know. I took this picture with my nikon d-40, using the 18-55 kit lens reversed. I really love the lighting, but it was a bit of a fluke. I used the on camera flash undiffused, but I think that the fact that I was so close caused most of the direct light to miss the subject, and instead hit a leaf and reflect back.


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Canteloupe

I’ve never had much success with melons. The problem, I believe, is a lack of sun. My garden is tucked under a pair of huge pecan trees, and, by all accounts, melons despise the shade. But this year it looks like I might finally get a lucky break, as one of cantaloupe vines has managed to put forth a single fruit. With a little good fortune, it will mature into a tasty melon. But, knowing my luck with these things, it will probably be abducted by aliens, spontaneously combust, or meet some other improbable fate.

My first cantaloupe, suspended high above the ground in a little burlap hammock.

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Mixing Chickens

A couple of weeks ago, while I was out of town, the barrier between the two groups of chickens came down. This was earlier than I had intended, and I was afraid that I was going to come home to quite a grizzly scene. On the contrary, they seem to have mixed quite well. I wouldn’t say that the big chickens LIKE the little ones, but they have tolerated them thus far. The biggest problem has been with the big ones eating the little ones’ food. I keep the feeder behind a barrier that filters out the big chickens, but before long they will all be the same size and this will be quite a bit more difficult. Though you are supposed to feed chicks starter until they pretty much start laying, I wouldn’t mind switching mine over to layer pellets before long (I did with my first batch and they turned out just fine), but they won’t go into the coop where the food is. They even sleep outside. I suppose that I will just start shoving them in the coop every night until they figure it out.

As these new chicks were straight run, about half of them have turned out to be roosters. The breeder is coming by soon to pick them up soon, but I want to keep them so bad! However, it has come to my attention that some people would be bothered by their early morning ruckus. Personally, I would prefer it over the sound of the dumpsters being emptied, but it would seem I am in the minority. Ah well.

A rooster (left) and hen (right)

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Good News And Bad News

The good news; I recently opened up my compost pile to find it full of rich, dark soil. The bad news; I also found a nest of yellow jackets in it.

Photo courtesy of Tate Lancaster

It is difficult to tell for sure, but I think that the nest is literally in the pile. Every time I try to turn it they start to swarm. I don’t really want to spray wasp killer in there, but I need to finish that compost up so that I can get another batch going. I can’t find the entrance hole anyway, so poison is pretty much ruled out. I’ll probably just have to wait for winter to come and kill the nest, as the only other option I can think of is to put on some thick clothes and dig out the nest. Any other suggestions would be welcome though.

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Cooperative Pseudoscorpions

While hiking in the Devil’s Millhopper State Park, I happened upon this intriguing scene playing out atop a fence post. A pair of wasps had been ensnared by a group of pseudoscorpions that occupied a crevice in the wood. Together, they had pinned the wasps down and were slowly killing them with venom injected through their claws. For a single pseudoscorpion, such prey would be far too dangerous. However, by cooperating, they had no problem taking down the wasps. I must admit, I found it far more interesting than the millhopper itself!

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